Control of protein glycosylation by culture medium supplementation and cell culture process parameters
US10676772B2 · kind B2 · utility
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10References
28Claims
0Family size
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Key dates
| Filing date | Apr 16, 2018 |
| Grant date | Jun 9, 2020 |
| Priority date | — |
| Expiry date | Apr 16, 2038 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2511/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention pertains to a cell culture medium comprising media supplements that are shown to control recombinant protein glycosylation and/or cell culture in a controlled or modulated (shifted) temperature to control recombinant protein glycosylation and/or cell culture with controlled or modulated seed density to control recombinant protein glycosylation, and methods of using thereof. The present invention further pertains to a method of controlling or manipulating glycosylation of a recombinant protein of interest in a large scale cell culture.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.