Semi-continuous culture methods
US10676775B2 · kind B2 · utility
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33Claims
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Key dates
| Filing date | Oct 14, 2015 |
| Grant date | Jun 9, 2020 |
| Priority date | — |
| Expiry date | Oct 14, 2035 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02E50/10
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided herein are methods of culturing a microorganism. The methods include providing a container comprising one or more microorganisms in a medium, which has a first carbon to nitrogen ratio; culturing the microorganisms until the culture reaches a threshold indicator; harvesting a portion of the culture while maintaining the majority of the culture in the container; and adding fresh medium comprising a second carbon to nitrogen ratio to the container with the majority of the culture comprising the microorganisms.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.