Patent · US Active

Compositions, methods and systems for polymerase chain reaction assays

US10676778B2 · kind B2 · utility

4Cited by

1References

20Claims

0Family size

Assignee

Bio-Rad Laboratories, Inc. · US

Inventors

Adam Lowe · Tracy, US
Geoff McDermott · Pleasanton, US
Christopher Hindson · Pleasanton, US
Erin R. Chia · Walnut Creek, US
Amy L. Hiddessen · Tracy, US
Benjamin Hindson · Pleasanton, US
Chunxiao Han · Dublin, US
Yaqi Wang · San Jose, US

Key dates

Filing date	Nov 20, 2017
Grant date	Jun 9, 2020
Priority date	—
Expiry date	Nov 20, 2037

Classification

Technology area (CPC B)Performing Operations; Transporting
CPC primaryB01L3/502784
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Methods, devices, systems and compositions for detecting nucleic acids in polymerase chain reaction assays, such as droplet digital polymerase chain reaction (ddPCR) assays, using intercalating dyes. A dual surfactant system with at least one fluorosurfactant and at least one non-ionic non-fluorosurfactant may be employed for droplet generation and nucleic acid detection.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.