Sample indexing for single cells

US10676779B2 · kind B2 · utility

Assignee

• Becton, Dickinson and Company · US

Inventors

• Christina Chang · Menlo Park, US
• Christina Fan · San Jose, US
• Eleen Shum · San Jose, US
• Jody Martin · Menlo Park, US
• Nidhanjali Bansal · Menlo Park, US
• James Ghadiali · San Diego, US
• Katherine Lazaruk · Redwood City, US
• Gretchen Yinbon Lam · Menlo Park, US

Key dates

• Filing date: Mar 27, 2018
• Grant date: Jun 9, 2020
• Expiry date: Mar 27, 2038

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2458/10
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Disclosed herein include systems, methods, compositions, and kits for sample identification. A sample indexing composition can comprise, for example, a protein binding reagent associated with a sample indexing oligonucleotide. Different sample indexing compositions can include sample indexing oligonucleotides with different sequences. Sample origin of cells can be identified based on the sequences of the sample indexing oligonucleotides. Sample indexing oligonucleotides can be barcoded using barcoded and lengthened using daisy-chaining primers.