Method of purifying albumin-fusion proteins

US10683340B2 · kind B2 · utility

Assignee

• MedImmune Limited · US

Inventors

• Timothy Pabst · Gaithersburg, US
• Mariko FONSECA · Gaithersburg, US
• Christopher Thompson · Gaithersburg, US
• Alan HUNTER · Gaithersburg, US
• Xiangyang Wang · Xi'an, CN
• Liu Tie · Gaithersburg, US
• Yiming Li · Lo Wu, CN

Key dates

• Filing date: Mar 11, 2016
• Grant date: Jun 16, 2020
• Expiry date: Mar 11, 2036

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/00
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The present invention relates to a method of purifying albumin-fusion proteins to reduce the level of oxidation of susceptible amino acid residues. The method comprises an affinity matrix chromatography step and an anion exchange chromatography step. The purified albumin-fusion proteins have low levels of oxidation and retain their enhanced half-life in vivo and its bioactivity. In some embodiments, the albumin-fusion protein comprises a scaffold, such as human Tenascin C scaffold. Compositions comprising the albumin-fusion protein are further disclosed.