Patent · US Active

Associating somatic gene mutations in glycophorin a with complex multifactorial diseases

US10684293B2 · kind B2 · utility

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26Claims
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Key dates

Filing dateMay 27, 2014
Grant dateJun 16, 2020
Priority date
Expiry dateJul 23, 2035

Classification

  • Technology area (CPC G)Physics
  • CPC primaryG01N2800/28
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

In the detection of a complex multifactorial disease, an MN blood group typing is performed on a first blood sample of a subject to be tested for a complex multifactorial disease, and on a second blood sample of a control subject having known phenotypes. Erythrocytes from the samples are isovolumetrically sphered, and M alleles and N alleles expressed on surfaces of erythrocyte cells of the first and second blood samples are labeled by contacting the blood samples with anti-M antibodies and anti-N antibodies associated with a marker. Labeled M alleles and labeled N alleles in the first and second blood samples are detected and quantified using the marker, in order to determine an incidence of somatic mutations in Glycophorin A of the patient. The incidence is compared with data pertaining to patients with a known complex multifactorial disease, to diagnose the patient.

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