CTLA4-binding protein peptide-linker masks

US10689423B2 · kind B2 · utility

Assignees

• City of Hope · US
• Thomas Jefferson University and Health System, Innovation Pillar · US

Inventors

• John C. Williams · Duarte, US
• Ulrich Rodeck · Philadelphia, US

Key dates

• Filing date: Jan 13, 2016
• Grant date: Jun 23, 2020
• Expiry date: Jan 13, 2036

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/74
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Provided herein are recombinant CTLA-4 binding proteins, which are, inter alia, useful for the treatment of cancer. The recombinant proteins provided herein are, inter alia, capable of binding CTLA-4 proteins on a tumor cell. In a first aspect, there is provided a recombinant CTLA-4 binding protein including (i) a CTLA-4 binding domain; (ii) a CTLA-4 binding domain masking peptide; and (iii) a cleavable peptide linker connecting the CTLA-4 binding domain masking peptide to the CTLA-4 binding domain. In another aspect, there is provided a dimerizing domain covalently attached to the CTLA-4 binding domain, wherein the binding protein domains are bound together.