Patent · US Active

Methods, compositions, and kits for detecting allelic variants

US10689694B2 · kind B2 · utility

2Cited by
47References
7Claims
0Family size

Assignee

Inventors

Key dates

Filing dateAug 22, 2018
Grant dateJun 23, 2020
Priority date—
Expiry dateAug 22, 2038

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12Q2600/156
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

In some embodiments, the present inventions relates generally to compositions, methods and kits for use in discriminating sequence variation between different alleles. More specifically, in some embodiments, the present invention provides for compositions, methods and kits for quantitating rare (e.g., mutant) allelic variants, such as SNPs, or nucleotide (NT) insertions or deletions, in samples comprising abundant (e.g., wild type) allelic variants with high specificity and selectivity. In particular, in some embodiments, the invention relates to a highly selective method for mutation detection referred to as competitive allele-specific TaqMan PCR (“cast-PCR”).

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.