Method for culturing pluripotent stem cells
US10696951B2 · kind B2 · utility
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8Claims
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Key dates
| Filing date | Sep 30, 2015 |
| Grant date | Jun 30, 2020 |
| Priority date | — |
| Expiry date | Sep 30, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2539/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided is a method for efficiently culturing pluripotent stem cells with higher safety. The present invention relates to a method for culturing pluripotent stem cells, the method comprising culturing an isolated pluripotent stem cells in a pseudo-microgravity environment to proliferate the pluripotent stem cells while maintaining the pluripotent stem cells in an undifferentiated state, thereby forming and growing spheroids of the pluripotent stem cells; and a method for inducing differentiation of pluripotent stem cells by using the method.
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