Nepetalactol oxidoreductases, nepetalactol synthases, and microbes capable of producing nepetalactone

US10696991B2 · kind B2 · utility

Assignee

• Zymergen Inc. · US

Inventors

• Grayson Wawrzyn · Oakland, US
• Christine Roche · Emeryville, US
• Patrick Westfall · Boulder, US
• Warren Lau · Emeryville, US
• Savita Ganesan · Emeryville, US
• Fern R. McSorley · Emeryville, US
• Zach Serber · Sausalito, US

Key dates

• Filing date: Aug 7, 2019
• Grant date: Jun 30, 2020
• Expiry date: Aug 7, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y103/01
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present disclosure provides isolated nepetalactone oxidoreductase polypeptides (NORs), nepetalactol synthases (NEPSs), and related polynucleotides, engineered host cells, and cultures, as well as methods for producing NORs and NEPSs, and for using them to produce nepetalactol, nepetalactone, and dihydronepetalactone. The present disclosure also provides methods for engineering cells (e.g., microbial cells) to produce nepetalactone from a fermentation substrate such as glucose, as well as engineered cells having this capability and related cultures and methods for producing nepetalactone.