Method and system for the production of recombinant proteins by cells

US10696997B2 · kind B2 · utility

Assignee

• UNIVERSITÉ LIBRE DE BRUXELLES · BE

Inventors

• Laurence Van Melderen · Waterloo, BE
• Baptiste J. S. Dumont · Brussels, BE
• Thibaut Hallaert · Waterloo, BE
• Gilles Vanwalleghem · Sint-Genesius-Rode, BE
• Philippe Goffin · Louvain-la-Neuve, BE

Key dates

• Filing date: Jul 6, 2015
• Grant date: Jun 30, 2020
• Expiry date: Aug 4, 2035

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/92
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A cassette sequence for the transformation of a host cell includes at least: a first nucleotide sequence encoding a peptide or protein of interest to be produced by the host cell. The first sequence is linked to a second nucleotide sequence providing resistance to a toxin or encoding an antitoxin peptide to the toxin. The nucleotide sequences are organized in such a way that production of the peptide encoded by the second nucleotide sequence(s) is translationally coupled to production of the peptide encoded by the first nucleotide sequence.