Amplification and detection of nucleic acids in a biological sample
US10697002B2 · kind B2 · utility
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18Claims
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Key dates
| Filing date | Nov 24, 2015 |
| Grant date | Jun 30, 2020 |
| Priority date | — |
| Expiry date | Aug 27, 2036 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02A50/30
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed are methods for identifying the presence or absence of a target nucleic acid from one or more organisms in a biological sample, said method comprising: (a) spinning the sample at a rotational velocity sufficient to pellet cellular debris and fluorescence inhibitors present within the sample and reduce fluorescence interference or quenching in the sample; and (b) directly amplifying and detecting the target nucleic acid in the sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.