Mutant 3-hydroxybutyrate dehydrogenase from Rhodobacter sphaeroides as well as methods and uses involving the same

US10704029B2 · kind B2 · utility

Assignee

• Roche Diabetes Care, Inc. · US

Inventors

• Daniela Beck · Penzberg, DE
• Mara Boenitz-Dulat · Tutzing, DE
• Peter Kratzsch · Penzberg, DE
• Thomas Streidl · Seeshaupt, DE
• Carina Horn · Biblis, DE

Key dates

• Filing date: Aug 9, 2018
• Grant date: Jul 7, 2020
• Expiry date: Dec 22, 2038

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y101/0103
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to a mutant 3-hydroxybutyrate dehydrogenase (3-HBDH) with improved performance relative to the wild-type 3-HBDH, a nucleic acid encoding the mutant 3-HBDH, a cell comprising the mutant 3-HBDH or the nucleic acid, a method of determining the amount or concentration of 3-hydroxybutyrate in a sample, and a device for determining the amount or concentration of 3-hydroxybutyrate in a sample.