Patent · US Active

DNA polymerase mutants with increased processivity of DNA synthesis

US10704031B2 · kind B2 · utility

4Cited by

1References

5Claims

0Family size

Assignee

Jiangnan University · CN

Inventors

Jing Wu · New York, US
Li Wang · Hangzhou City, CN

Key dates

Filing date	Nov 22, 2018
Grant date	Jul 7, 2020
Priority date	—
Expiry date	Nov 22, 2038

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Y207/07007
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Disclosed are Dpo4 DNA polymerase mutants with increased DNA synthesis processivity. The mutant enzymes, Dpo4 A181D and Dpo4 E63K, are constructed based on the wild-type Dpo4 derived from Sulfolobus solfataricus to obtain Dpo4DNA polymerase mutants with increased DNA processivity. The extension length of Dpo4 A181D and Dpo4 E63K are respectively increased by 25% and 18.8% than that of the wild-type Dpo4. The fidelity of Dpo4 A181D and wild-type Dpo4 are similar, and the fidelity of Dpo4 E63K is increased from that of the wild-type Dpo4. In summary, the mutants Dpo4 A181D and Dpo4 E63K obtained by the present invention have increased processivity compared to that of the wild-type Dpo4 DNA polymerase.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.