Ligation-based RNA amplification

US10704042B2 · kind B2 · utility

Assignee

• GE Healthcare Bio-Science AB · US

Inventors

• John Richard Nelson · Fryeburg, US
• R. Duthie · Schenectady, US
• Rohini Dhulipala · Kendall Park, US
• Gregory Andrew Grossman · Halfmoon, US
• Anuradha Sekher · Niskayuna, US

Key dates

• Filing date: Apr 30, 2018
• Grant date: Jul 7, 2020
• Expiry date: Jun 30, 2038

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6865
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods of amplification, purification and detection of nucleic acid sequences especially RNA are described. One aspect of the method involves the hybridisation and subsequent ligation of a nucleic acid structure to the nucleic acid sequence desired to be manipulated. The methods require that the nucleic acid structure comprises a double stranded region and a single stranded region. The single stranded region is complementary to the RNA sequence of interest. The double stranded region may also contain additional functionalities which are then used subsequently in the method.