Method for preparing magnetic microsphere for separation of biological protein and use thereof
US10705079B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 29, 2014 |
| Grant date | Jul 7, 2020 |
| Priority date | — |
| Expiry date | Aug 17, 2034 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/54333
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Disclosed is a method for preparing a magnetic microsphere for the separation of biological proteins. A magnetic microsphere matrix is treated by formulating and using an appropriate emulsified liquid, and modification of the surface of the magnetic microsphere matrix is realized by emulsion polymerization, thereby obtaining a magnetic microsphere coated with a polyacrylate polymer layer. Said emulsified liquid comprises the following components therein: acyclic acid monoester compounds, acyclic acid glycol compounds, initiators and optionally anionic surface active agents and water. The magnetic microsphere significantly reduces the non-specific adsorption of other proteins, without affecting the joining ability for a specific protein, when used in the separation of biological proteins. A new selection is provided to realize the separation engineering of high protein specificity adsorption.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.