Salmonella paratyphi A with an O-antigen having an extended carbohydrate chain and use thereof

US10709777B2 · kind B2 · utility

Assignee

• Institute of Biotechnology, Academy of Military Medical Sciences, China · CN

Inventors

• Jun Wu · Beijing, CN
• Peng Sun · Beijing, CN
• Bo Liu · Beijing, CN
• Shaohong Chang · Beijing, CN
• Xin Gong · Qingdao, CN
• Hengliang Wang · Beijing, CN
• Li Zhu · Burbank, US
• Chao-Chi Pan · Hsinchu, TW
• Erling Feng · Beijing, CN

Key dates

• Filing date: Sep 7, 2015
• Grant date: Jul 14, 2020
• Expiry date: Sep 7, 2035

Classification

• Technology area (CPC —): General

Abstract

The present invention discloses a Salmonella paratyphi A with an O-antigen having an extended carbohydrate chain and uses thereof. The method comprises the following steps: inactivating an cld gene encoding an enzyme controlling chain length of O-antigen of a Salmonella paratyphi A strain to obtain a Salmonella paratyphi A with deletion of cld gene; allowing overexpression of cldLT2 gene encoding an enzyme controlling chain length of O-antigen of Salmonella typhimurium in Salmonella paratyphi A deficient in the cld gene encoding an enzyme controlling chain length of O-antigen, so as to extend carbohydrate chain length of O-antigen. Both of the Salmonella paratyphi A O-polysaccharide-recombinant fusion protein conjugate vaccines rCTB45733-OPSSpty50973 and rEPA4573-OPSSpty50973 as prepared by using Salmonella paratyphi A with an O-antigen having an extended carbohydrate chain can induce mice to generate specific antibodies against Salmonella paratyphi A, and their antibody titers are significantly improved.