System and method for assessing cellular metabolic activity
US10712272B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 27, 2017 |
| Grant date | Jul 14, 2020 |
| Priority date | — |
| Expiry date | Jul 8, 2038 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG06T2207/10064
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods and corresponding apparatus and systems for assessing cellular metabolic activity are disclosed. In one aspect, a cell can be illuminated with optical radiation in order to cause multi-photon excitation of at least one endogenous metabolic cofactor in that cell and cause the excited metabolic cofactor to emit fluorescent radiation. A detector can be used to detect the fluorescent radiation emitted by the excited endogenous metabolic cofactor. A computer processor can analyze the fluorescent radiation to derive the following parameters: (1) using a computer processor to analyze the intensity of the fluorescent radiation, (2) a fluorescence lifetime of at least one of the excited metabolic cofactor, (3) a parameter indicative of mitochondrial clustering in the cell. These parameters can be used to assess at least one metabolic process of the cell.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.