Stabilization of poly(A) sequence encoding DNA sequences

US10717982B2 · kind B2 · utility

Assignees

• BIONTECH RNA PHARMACEUTICALS GMBH · DE
• TRON—Translationale Onkologie an der Universitätsmedizin der Johannes Gutenberg-Universität Mainz · DE

Inventors

• Florian Eberle · Dossenheim, DE
• Ugur Sahin · Mainz, DE
• Andreas Kuhn · Mainz, DE
• Britta Vallazza · Hagen, DE
• Mustafa Diken · Mainz, DE

Key dates

• Filing date: Jul 6, 2015
• Grant date: Jul 21, 2020
• Expiry date: Sep 26, 2035

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2830/50
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The present invention relates to nucleic acid molecules containing poly (dA:dT) regions which are stabilized in E. coli, methods of propagating such nucleic acid molecules in E. coli, methods of obtaining RNA, peptides or proteins using such nucleic acid molecules and to RNA which is obtained from such nucleic acid molecules and its use. In particular, the poly (dA:dT) regions contain at least one disruption by a sequence not encoding a sequence solely composed of A residues.