Single molecule electronic multiplex SNP assay and PCR analysis

US10718011B2 · kind B2 · utility

Assignee

• The Trustees of Columbia University in the City of New York · US

Inventors

• Chuanjuan Tao · Fort Lee, US
• Shiv Kumar · Montgomery, US
• Minchen Chien · Tenafly, US
• Jingyue Ju · Englewood Cliffs, US

Key dates

• Filing date: Feb 12, 2015
• Grant date: Jul 21, 2020
• Expiry date: Dec 7, 2035

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2565/631
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

This invention provides methods of using labeled primers or probes for nucleic acid target detection and to detect the identity or presence of a nucleotide at certain positions in nucleic acid sequences with single molecule sensitivity using nanopore detection, and sets of oligonucleotide primers for use in such methods, as well as methods of quantitative PCR coupled with nanopore detection.