Method for detecting fluorescent species that are reversibly photoswitchable at a high frequency
US10718712B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Aug 10, 2017 |
| Grant date | Jul 21, 2020 |
| Priority date | — |
| Expiry date | Aug 10, 2037 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2021/6441
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method is provided for detecting a fluorescent species that is reversibly photoswitchable at high frequency, and more precisely to a method for detecting at least one reversibly photoswitchable fluorescent species, including a step consisting in illuminating a sample containing a reversibly photoswitchable fluorescent species with a first illuminating light beam, of wavelength λ1, and periodically modulated at an angular frequency ω, and with a second illuminating light beam of wavelength λ2 different from λ1, which is periodically modulated at the angular frequency ω, the second illuminating light beam being modulated in antiphase with respect to the first illuminating light beam.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.