Microfluidic cell culture

US10723988B2 · kind B2 · utility

Assignee

• Berkeley Lights, Inc. · US

Inventors

• Randall D. Lowe, Jr. · Emeryville, US
• Kristin G. Beaumont · New York, US
• Aathavan Karunakaran · Berkeley, US
• Natalie C. Marks · Albany, US
• Jason M. McEwen · Corona, US
• Mark P. White · San Francisco, US
• J. Tanner Nevill · El Cerrito, US
• Gang F. Wang · Mountain View, US
• Andrew W. McFarland · Berkeley, US
• Daniele Malleo · Corona, US
• Keith J. Breinlinger · Livermore, US
• Xiao Guan · Malden, US
• Kevin T. Chapman · Emeryville, US

Key dates

• Filing date: Apr 22, 2016
• Grant date: Jul 28, 2020
• Expiry date: Aug 20, 2036

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12M29/10
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.