Reducing DNA damage during sample preparation and sequencing using siderophore chelators

US10724075B2 · kind B2 · utility

Assignee

• Illumina Cambridge Limited · GB

Inventors

• Vincent Peter Smith · Saffron Walden, GB
• Sohela de Rozieres · San Diego, US

Key dates

• Filing date: Oct 19, 2017
• Grant date: Jul 28, 2020
• Expiry date: Jun 1, 2038

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6869
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Embodiments disclosed herein provide reagents and kits for nucleic acid preparation comprising a siderophore. Embodiments disclosed herein provide methods for preparing a nucleic acid library, which comprise: providing a plurality of nucleic acid molecules from a sample; and manipulating the plurality of nucleic acid molecules in a reagent for nucleic acid preparation comprising a siderophore. Further, embodiments disclosed herein provide methods for reducing oxidative damage to a nucleic acid molecule or increasing the Q (phred) score of a sequencing reaction, which methods comprise preparing the nucleic acid molecule in the absence of EDTA.