Methods for analyzing DNA
US10724082B2 · kind B2 · utility
16Cited by
578References
14Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Dec 8, 2017 |
| Grant date | Jul 28, 2020 |
| Priority date | — |
| Expiry date | Mar 16, 2038 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/686
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention generally relates to methods for increasing the amount of DNA available for analysis when using partitioned samples and parallel processing. For example, double-stranded DNA can be dissociated into two single-stranded components, and the single strands partitioned into different droplets prior to analysis. The disclosed methods are useful for performing digital PCR analysis on samples where the target DNA is not in abundance, for example when the sample originates from a body fluid or an FFPE sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.