Methods and systems for analysis of chromatin

US10725027B2 · kind B2 · utility

Assignee

• 10x Genomics, Inc. · US

Inventors

• Jason Bell · Palo Alto, US
• Geoffrey McDermott · Livermore, US
• Francesca Meschi · Pleasanton, US
• Michael Schnall-Levin · San Francisco, US
• Xinying Zheng · Pleasanton, US

Key dates

• Filing date: Apr 4, 2019
• Grant date: Jul 28, 2020
• Expiry date: Apr 4, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C40B70/00
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from a single cell. Such polynucleotide processing may be useful for a variety of applications. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins) and chromatin (e.g., accessible chromatin).