Homoallylamines as formaldehyde-responsive triggers
US10730892B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 18, 2016 |
| Grant date | Aug 4, 2020 |
| Priority date | — |
| Expiry date | Nov 9, 2036 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N15/1459
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Probes for formaldehyde (FA) including a homoallylamine trigger group attached to a detectable moiety are provided. Aspects of the probes include luminogenic or fluorogenic probes, such as a probe including a quencher in energy-receiving proximity to a fluorophore. Also provided are methods of using the probes for sensitive and bio orthogonal detection of FA in a sample. Aspects of the methods include selectively reacting the probe with the formaldehyde in the sample to a release (e.g., via a 2-aza-Cope rearrangement) a reporter group comprising a detectable moiety. Aspects of the methods detecting formaldehyde in a cell, tissue, organ or fluid in a subject. Also provided are compositions and kits including the subject probes that find use in practicing various embodiments of the subject methods.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.