Method and devices for the in vitro production of arrangements of cell layers

US10731119B2 · kind B2 · utility

Assignees

• UNIVERSITÄTSKLINIKUM JENA · DE
• Microfluidic Chipshop GmbH · DE

Inventors

• Alexander Mosig · Jena, DE
• Knut Rennert · Jena, DE

Key dates

• Filing date: May 7, 2015
• Grant date: Aug 4, 2020
• Expiry date: Dec 12, 2035

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2525/00
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention is directed to a method for the in vitro production of arrangements of cell layers in which a first well (2.1) which is closed off from its environment except for a first inlet opening (4.1) and a first outlet opening (5.1) and which has as a first cell substrate (6.1) a first wall (2.1.1) and as a second cell substrate (6.2) an opposite, second wall (2.1.2) which is separated from the first wall (2.1.1) by a first gap, a free surface of a cell substrate (6.1, 6.2) to be colonized with cells is oriented orthogonal to the Earth's gravitational force, and cells (9) are adhered to the cell substrate (6.1, 6.2) to be colonized. The invention is further directed to a method of maintaining the biological functionalities of the cell layers and semi-finished products of a device for the in vitro production and culturing of cell layers and a method for the production of the device.