Regeneration of a functional pulmonary vascular bed
US10731135B2 · kind B2 · utility
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6Claims
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Key dates
| Filing date | Sep 9, 2016 |
| Grant date | Aug 4, 2020 |
| Priority date | — |
| Expiry date | Sep 9, 2036 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2533/92
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for vascular regeneration comprises delivering endothelial cells to a lung scaffold, delivering perivascular cells to the lung scaffold, and providing a multiphase culture program to the scaffold. The multiphase culture program comprises a first phase including delivering an angiogenic medium, e.g., having 40-100 ng/ml of pro-angiogenic factors, and a second phase including delivering a stabilization medium, e.g., having 0.5-2% of serum and 1-20 ng/ml of angiogenic factors.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.