Nucleic acid probe and method of detecting genomic fragments

US10731214B2 · kind B2 · utility

Assignee

• VANADIS DIAGNOSTICS · SE

Inventors

• Carl Oscar Fredrik Dahl · Sigtuna, SE
• Olof John Ericsson · Uppsala, SE

Key dates

• Filing date: Jan 17, 2019
• Grant date: Aug 4, 2020
• Expiry date: Jan 17, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6816
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Provided herein, among other things, is a method of processing a nucleic acid sample. In some embodiments, the method comprises a) hybridizing a sample comprising a target fragment to a nucleic acid probe comprising: i. a head sequence and a tail sequence, wherein the head and tail sequences are at the ends of a first oligonucleotide molecule; and ii. a splint sequence comprising, in order: an upstream flanking sequence that is complementary to the head sequence; a target complementary sequence that is complementary to the target fragment; and a downstream flanking sequence that is complementary to the tail sequence; thereby producing a hybridization product in which the ends of the target fragment are ligatably adjacent to the ends of the head and tail sequences in the first oligonucleotide molecule; and b) ligating the ends of the target fragment to the ends of the head and tail sequences of the first oligonucleotide molecule, thereby producing a cyclic product that comprises the target fragment and the head and tail sequences. Probes and kits for performing the method are also provided.