Method for determining the presence or absence of methylation in a sample

US10731215B2 · kind B2 · utility

Assignee

• EPIGENOMICS AG · DE

Inventors

• Matthias Ballhause · Berlin, DE
• Kurt Berlin · Stahnsdorf, DE
• Theo De Vos · Seattle, US
• Dimo Dietrich · Berlin, DE
• Volker Liebenberg · Berlin, DE
• Catherine E. Lofton-Day · Brier, US
• Joe Lograsso · Seattle, US
• Jennifer Maas · Seattle, US
• Fabian Model · Berlin, DE
• Matthias Schuster · Weinheim, DE
• Andrew Z. Sledziewski · Shoreline, US
• Reimo Tetzner · Berlin, DE

Key dates

• Filing date: Apr 17, 2006
• Grant date: Aug 4, 2020
• Expiry date: Oct 15, 2029

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/143333
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Aspects of the present invention relate to compositions and methods for providing DNA fragments from a remote sample. In particular aspects a remote sample comprising DNA is provided, DNA is isolated from the remote sample, and the isolated DNA is treated in a way which allows differentiation of methylated and unmethylated cytosine. Additional, particular embodiments provide compositions and methods for methylation analysis of DNA derived from a remote sample. Other aspects provide for compositions and methods of whole genome amplification of bisulfite treated DNA. Other aspects provide methods for determining the presence or absence of methylation of at least one cytosine, or a series of cytosines in cis, in human DNA of a blood sample, a plasma sample, a serum sample or a urine sample from a human individual.