Tevi chimeric endonuclease and their preferential cleavage sites
US10738289B2 · kind B2 · utility
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17Claims
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Key dates
| Filing date | Mar 22, 2017 |
| Grant date | Aug 11, 2020 |
| Priority date | — |
| Expiry date | Mar 22, 2037 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/80
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a method to cleave target nucleic acid sequence by the catalytic domain of a GIY-YIG homing endonucleases I-TevI. More precisely, the invention relates to the deciphering of new preferential I-TevI cleavage sites for efficient and specific cleavage activity. The invention concerns a method for the generation of TevI specific chimeric endonucleases to target nucleic acid sequence including such cleavage sites and methods of using same for gene editing.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.