Patent · US Active

Method for separating DNA by size

US10745686B2 · kind B2 · utility

0Cited by

17References

43Claims

0Family size

Assignee

QIAGEN GmbH · DE

Inventors

Tanya SPERLING · Hilden, DE
Nicola Scholle · Hilden, DE
Thorsten Singer · Solingen, DE
Margit Hiesinger · Hilden, DE

Key dates

Filing date	Feb 7, 2014
Grant date	Aug 18, 2020
Priority date	—
Expiry date	Jan 22, 2035

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q1/6869
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present invention provides method for isolating DNA molecules having a size above a certain cut-off value from a DNA containing sample, comprising a) contacting the sample with a binding buffer which comprises a chaotropic agent and a buffering agent to provide a binding mixture and binding DNA molecules having a size above the cut-off value to a binding matrix which has a silicon containing surface, wherein the cut-off value is determined by the pH value of the binding mixture; b) separating the bound DNA from the remaining sample; c) optionally washing the bound DNA; and d) optionally eluting the bound DNA from the binding matrix. Said method allows the size selective purification of DNA molecules.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.