Production method for charged non-protein amino acid-containing peptide
US10745692B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Aug 4, 2014 |
| Grant date | Aug 18, 2020 |
| Priority date | — |
| Expiry date | Feb 29, 2036 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02P20/55
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An object of the present invention is to provide a method of producing a peptide containing a charged non-proteinogenic amino acid in a cell-free translation system, and the like.The present invention provides a method of producing a peptide containing a charged non-proteinogenic amino acid. It includes a step of expressing a peptide in a cell-free translation system including (i) at least one tRNA to which a non-proteinogenic amino acid having a protecting-group-introduced charged group has been bound and (ii) a nucleic acid that encodes the peptide and contains at least one codon corresponding to an anticodon of the tRNA; and a step of removing the protecting group of the non-proteinogenic amino acid residue contained in the peptide.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.