Cell barcoding in microfluidics

US10745741B2 · kind B2 · utility

Assignee

• European Molecular Biology Laboratory · DE

Inventors

• Ramesh Utharala · Miryalaguda, IN
• Chawaree Chaipan · Heidelberg, DE
• Lukas Nögel · Heidelberg, DE
• Christoph Merten · Heidelberg, DE

Key dates

• Filing date: Jun 27, 2016
• Grant date: Aug 18, 2020
• Expiry date: Oct 21, 2036

Classification

• Technology area (CPC B): Performing Operations; Transporting
• CPC primary: B01L2400/06
• WIPO field: Chemical engineering
• WIPO sector: Chemistry

Abstract

The present invention relates to the field of microfluidics and in particular to methods for co-localizing a particle comprising DNA and/or RNA with a known barcode oligonucleotide. Thereby, the transcriptome or DNA of a cell can be barcoded and correlated to a cell phenotype or examined for the effect of a drug on the cell. The invention also provides microfluidic devices and systems having properties which make them particularly suitable for use in the methods of the invention.