Methods for detecting nucleic acid sequence variants
US10745749B2 · kind B2 · utility
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16Claims
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Key dates
| Filing date | Oct 30, 2017 |
| Grant date | Aug 18, 2020 |
| Priority date | — |
| Expiry date | Oct 30, 2037 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/112
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides methods for detecting the presence or absence of a nucleic acid variant in a target region. These methods include amplifying the target region with a forward primer and a reverse primer in the presence of a selector blocker. The selector blocker includes a sequence complementary to the target region in the absence of the nucleic acid variant. The methods further include detecting amplification of the target region where amplification of the target region indicates the presence of the nucleic acid variant in the target region. The nucleic acid variant can include deletions, mutations or insertions.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.