Production of manool

US10752922B2 · kind B2 · utility

Assignee

• FIRMENICH SA · CH

Inventors

• Michel Schalk · Collonges-sous-Salève, FR
• Laurent Daviet · Geneva, CH
• Letizia Rocci · Satigny, CH
• Daniel Solis Escalante · Satigny, CH

Key dates

• Filing date: Dec 18, 2017
• Grant date: Aug 25, 2020
• Expiry date: Dec 18, 2037

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y505/01012
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Described herein are methods of producing (+)-manool, the methods including: contacting geranylgeranyl diphosphate with a copalyl diphosphate (CPP) synthase to form a (9S, 10S)-copalyl diphosphate and contacting the CPP with a sclareol synthase enzyme to form (+)-manool and derivatives thereof. Also described herein are nucleic acids encoding CPP synthases and sclareol synthases for use in the methods. Further described herein are expression vectors and non-human host organisms and cells including nucleic acids encoding a CPP synthase and a sclareol synthase as described herein.