Methods of lowering the error rate of massively parallel DNA sequencing using duplex consensus sequencing

US10752951B2 · kind B2 · utility

Assignee

• University of Washington through its Center for Commercialization · US

Inventors

• Jesse Salk · Seattle, US
• Lawrence A. Loeb · Bellevue, US
• Michael Schmitt · Seattle, US

Key dates

• Filing date: Jul 17, 2019
• Grant date: Aug 25, 2020
• Expiry date: Jul 17, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6869
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Next Generation DNA sequencing promises to revolutionize clinical medicine and basic research. However, while this technology has the capacity to generate hundreds of billions of nucleotides of DNA sequence in a single experiment, the error rate of approximately 1% results in hundreds of millions of sequencing mistakes. These scattered errors can be tolerated in some applications but become extremely problematic when “deep sequencing” genetically heterogeneous mixtures, such as tumors or mixed microbial populations. To overcome limitations in sequencing accuracy, a method Duplex Consensus Sequencing (DCS) is provided. This approach greatly reduces errors by independently tagging and sequencing each of the two strands of a DNA duplex. As the two strands are complementary, true mutations are found at the same position in both strands. In contrast, PCR or sequencing errors will result in errors in only one strand. This method uniquely capitalizes on the redundant information stored in double-stranded DNA, thus overcoming technical limitations of prior methods utilizing data from only one of the two strands.