Patent · US Active

Method for detecting mutations in single cells or single molecules

US10752954B2 · kind B2 · utility

0Cited by

4References

13Claims

0Family size

Assignee

The Trustees of the University of Pennsylvania · US

Inventors

Arjun Raj · Lansdowne, US
Marshall J. Levesque · Philadelphia, US

Key dates

Filing date	Mar 13, 2014
Grant date	Aug 25, 2020
Priority date	—
Expiry date	Jun 20, 2034

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q2600/156
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The invention provides a high efficiency fluorescence in situ hybridization (FISH) method for detecting mutations on individual RNA transcripts, including both exonic and intronic RNA transcripts. In certain embodiments, the method is used to quantify allelic expression at the population and single cell level, and also to distinguish maternal chromosomes from paternal chromosomes in single cells.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.