Compositions and methods for enrichment of cells

US10758573B2 · kind B2 · utility

Assignees

• President and Fellows of Harvard College · US
• CHARITÉ—UNIVERSITÄTSMEDIZIN BERLIN · DE

Inventors

• Pascal Joly · Boston, US
• Georg Duda · Berlin, DE
• Thomas E. Schaus · Arlington, US
• Anke Dienelt · Berlin, DE
• Andrea Sass · Berlin, DE
• David J. Mooney · Ann Arbor, US

Key dates

• Filing date: Jun 9, 2016
• Grant date: Sep 1, 2020
• Expiry date: Aug 15, 2036

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2310/3517
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The present invention is directed to a device for enriching cells with a cell surface marker, comprising an aptamer suitable for specifically binding the cell surface marker, and beads coupled thereto, wherein the aptamer is coupled to the beads in a manner that allows for release of cells expressing the cell surface marker, in the absence of a chemical agent, and production of a cell population enriched for cells expressing the cell surface marker, substantially free of beads and aptamer. Kits comprising the device or components thereof, and methods of cell enrichment, are also provided. In exemplary embodiments, the device contains an aptamer that specifically binds CD31.