Modified template-independent enzymes for polydeoxynucleotide synthesis
US10760063B2 · kind B2 · utility
3Cited by
1References
25Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Aug 27, 2018 |
| Grant date | Sep 1, 2020 |
| Priority date | — |
| Expiry date | Aug 27, 2038 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P19/34
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention includes methods for identifying polymerases, such as modified terminal nucleotidyl transferases (TdT), that are capable of binding nucleotides comprising removable 3′-O-blocking moieties to a nucleic acid initiator, without the use of a template. The invention further includes the identified polymerases, and methods of using the polymerases for de novo synthesis of predetermined oligonucleotide sequences.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.