Composition for detection of RNA
US10760074B2 · kind B2 · utility
1Cited by
11References
11Claims
0Family size
Assignee
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Key dates
| Filing date | Aug 31, 2009 |
| Grant date | Sep 1, 2020 |
| Priority date | — |
| Expiry date | Feb 13, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6848
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A composition for use in amplifying cDNA synthesized by a reverse transcription reaction and detecting RNA that serves as a template of the reverse transcription reaction, the composition containing a thermostable DNA polymerase, a thermostable ribonuclease H, and an intercalating dye. Since the composition of the present invention can suppress the influences to the nucleic acid amplification reaction by RNA that serves as a template for cDNA synthesis, the composition is useful in the detection of RNA, and more useful in quantification of RNA having a desired sequence by real-time RT-PCR.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.