High-temperature neutral cellulase as well as coding gene and application thereof

US10767170B2 · kind B2 · utility

Assignee

• Feed Research Institute, Chinese Academy of Agricultural Sciences · CN

Inventors

• Bin Yao · Nanhu, CN
• Pengjun Shi · Chengdu, CN
• Hong Yang · Suzhou, CN
• Huoqing Huang · Beijing, CN
• Huiying Luo · Beijing, CN
• Peilong Yang · Beijing, CN
• Yaru Wang · Beijing, CN
• Xiaoyun Su · Beijing, CN
• Yingguo Bai · Beijing, CN
• Xia Shi · Beijing, CN
• Rui Ma · Naperville, US
• Kun Meng · Beijing, CN

Key dates

• Filing date: Dec 9, 2014
• Grant date: Sep 8, 2020
• Expiry date: May 24, 2036

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y302/01004
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Provided are a fungus-sourced high-temperature neutral Family-45 cullulase as well as a coding gene and application thereof. The cullulase has optimal pH value of 5.5, and optimal temperature of 60° C., has certain enzyme activity in alkaline condition, and has good alkali resistance, maintains about 70% enzyme activity in optimal condition after being processed at 90° C. for 1 hour, maintains about 50% enzyme activity in optimal condition after being processed in boiling water for 1 hour, and can be well applied in c and other fields.