High specificity genome editing using chemically modified guide RNAs
US10767175B2 · kind B2 · utility
20Cited by
6References
23Claims
0Family size
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Key dates
| Filing date | Apr 20, 2017 |
| Grant date | Sep 8, 2020 |
| Priority date | — |
| Expiry date | Apr 20, 2037 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2320/50
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to guide RNAs having chemical modifications and their use in CRISPR-Cas systems. The chemically modified guide RNAs have enhanced specificity for target polynucleotide sequences. The present invention also relates to methods of using chemically modified guide RNAs for cleaving or nicking polynucleotides, and for high specificity genome editing.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.