CRISPR-based compositions and methods of use
US10767176B2 · kind B2 · utility
7Cited by
3References
1Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Oct 21, 2016 |
| Grant date | Sep 8, 2020 |
| Priority date | — |
| Expiry date | Oct 21, 2036 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2320/53
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention pertains to modified compositions for use in CRISPR systems, and their methods of use. In particular, length-modified and chemically-modified forms of crRNA and tracrRNA are described for use as a reconstituted guide RNA for interaction with Cas9 of CRISPR systems. The resultant length-modified and chemically-modified forms of crRNA and tracrRNA are economical to produce and can be tailored to have unique properties relevant to their biochemical and biological activity in the context of the CRISPR Cas9 endonuclease system.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.