Engineering an increase in ethanol production by altering cofactor specificity
US10767196B2 · kind B2 · utility
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9Claims
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Key dates
| Filing date | Nov 30, 2012 |
| Grant date | Sep 8, 2020 |
| Priority date | — |
| Expiry date | Nov 30, 2032 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02E50/10
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides for the manipulation of cofactor usage in a recombinant host cell to increase the formation of desirable products. In some embodiments, the invention provides for a recombinant microorganism comprising a mutation in one or more native enzymes such that their cofactor specificity is altered in such a way that overall cofactor usage in the cell is balanced for a specified pathway and there is an increase in a specific product formation within the cell. In some embodiments, endogenous enzymes are replaced by enzymes with an alternate cofactor specificity from a different species.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.