Multivalent binding composition for nucleic acid analysis

US10768173B1 · kind B1 · utility

Assignee

• Element Biosciences, Inc. · US

Inventors

• Sinan ARSLAN · San Diego, US
• Molly He · San Diego, US
• Matthew KELLINGER · San Diego, US
• Jake LEVIEUX · San Diego, US
• Michael Previte · San Diego, US
• Junhua ZHAO · San Diego, US
• Su-Chun Zhang · Middleton, US

Key dates

• Filing date: Sep 23, 2019
• Grant date: Sep 8, 2020
• Expiry date: Sep 23, 2039

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/582
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Multivalent binding compositions including a particle-nucleotide conjugate having a plurality of copies of a nucleotide attached to the particle are described. The multivalent binding compositions allow one to localize detectable signals to active regions of biochemical interaction, e.g., sites of protein-protein interaction, protein-nucleic acid interaction, nucleic acid hybridization, or enzymatic reaction, and can be used to identify sites of base incorporation in elongating nucleic acid chains during polymerase reactions and to provide improved base discrimination for sequencing and array based applications.