Patent · US Active

Oplophorus-derived luciferases, novel coelenterazine substrates, and methods of use

US10774364B2 · kind B2 · utility

3Cited by

43References

9Claims

0Family size

Assignee

Promega Corporation · US

Inventors

Brock Binkowski · Sauk City, US
Lance P. Encell · Fitchburg, US
Mary Hall · Waunakee, US
Matthew Robers · Madison, US
Michael R. Slater · Madison, US
Keith V. Wood · Verona, US
Monika G. Wood · Verona, US

Key dates

Filing date	Apr 9, 2018
Grant date	Sep 15, 2020
Priority date	—
Expiry date	Apr 9, 2038

Classification

Technology area (CPC A)Human Necessities
CPC primaryA01K2217/072
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.