Patent · US Active

Methods for preparing a sample for nucleic acid amplification using tagmentation

US10774367B2 · kind B2 · utility

24Cited by
2References
20Claims
0Family size

Assignee

Inventors

Key dates

Filing dateOct 17, 2018
Grant dateSep 15, 2020
Priority date—
Expiry dateNov 5, 2038

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12Q1/6846
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

Presented are methods and compositions for preparing samples for amplification and sequencing. Particular embodiments relate to methods of preparing nucleic acid-containing cellular samples for library amplification, wherein the methods include providing nucleic acid containing-cellular samples from blood or FFPE samples, lysing cells of the sample to liberate nucleic acids, and performing tagmentation without purifying the liberated nucleic acids.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.