Methods for multiplex PCR amplification of target loci in a nucleic acid sample

US10774380B2 · kind B2 · utility

Assignee

• Natera, Inc. · US

Inventors

• Allison Ryan · Redwood City, US
• Styrmir Sigurjonsson · San Jose, US
• Milena Banjevic · Los Altos Hills, US
• George Gemelos · Walnut, US
• Matthew Hill · Belmont, US
• Johan Baner · San Francisco, US
• Matthew Rabinowitz · San Francisco, US
• Zachary Demko · San Francisco, US

Key dates

• Filing date: Feb 27, 2019
• Grant date: Sep 15, 2020
• Expiry date: Feb 27, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/156
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods for non-invasive prenatal paternity testing are disclosed herein. The method uses genetic measurements made on plasma taken from a pregnant mother, along with genetic measurements of the alleged father, and genetic measurements of the mother, to determine whether or not the alleged father is the biological father of the fetus. This is accomplished by way of an informatics based method that can compare the genetic fingerprint of the fetal DNA found in maternal plasma to the genetic fingerprint of the alleged father.