Multifunctional nanoparticle bioconjugates for photoacoustic-based recording of cellular membrane potential

US10780185B2 · kind B2 · utility

Assignee

• The Government of the United States of America, as represented by the Secretary of the Navy · US

Inventors

• James B. Delehanty · Washington, US
• Michael H. Stewart · Springfield, US
• Okhil Nag · Alexandria, US
• Jeffrey R. Deschamps · Laurel, US
• Kimihiro Susumu · Alexandria, US
• Eunkeu Oh · Washington, US
• Lauren D. Field · College Park, US
• Alexander L. Efros · Annandale, US
• Alan L. Huston · Springfield, US
• Igor L. Medintz · Springfield, US
• Philip E. Dawson · Solana Beach, US
• Nashaat Rasheed · Fairfax, US
• Parag V. Chitnis · Fairfax, US
• John R. Cressman · Fairfax, US

Key dates

• Filing date: Jul 10, 2018
• Grant date: Sep 22, 2020
• Expiry date: Feb 22, 2039

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/588
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A construct for detecting cellular membrane potential includes a nanoparticle operable as an electron donor; a modular peptide attached to the nanoparticle, the peptide comprising a nanoparticle association domain, a motif configured to mediate peptide insertion into the plasma membrane, and at least one attachment point for an electron acceptor positioned at a controlled distance from the nanoparticle; and an electron acceptor. The nanoparticle can be a quantum dot and the electron acceptor can be C60 fullerene. Photoacoustic emission from the construct correlates with cellular membrane potential.